Role of Slug transcription factor in human mesenchymal stem cells.
"We used human MSCs from tibial plateau (TP) trabecular bone, iliac crest (IC) bone marrow and Wharton's jelly (WJ) umbilical cord demonstrating a variability in their mineral matrix deposition, and in the expression levels of TFs including Runx2, Sox9, Sox5, Sox6, STAT1 and Slug, all involved in the control of osteochondroprogenitors differentiation program. Because we reasoned that the basal expression level of some TFs with crucial role in the control of MSC fate may be correlated with osteogenic potential, we considered the possibility to affect the hMSCs behaviour by using gene silencing approach without exposing cells to induction media. Slug-silenced cells changed in morphology, decreased in their migration ability, increased Sox9 and Sox5 and decreased Sox6 and STAT1 expression. On the contrary, the effect of Slug depletion on Runx2 was influenced by cell type. Interestingly, we demonstrated a direct in vivo regulatory action of Slug by chromatin immunoprecipitation, showing a specific recruitment of this TF in the promoter of Runx2 and Sox9 genes."
"Slug-repressed cells had an impaired ability to close the wounded area compared with control and scrambled cells. After 48 hrs, gap closure in Slug-repressed hMSCs was significantly reduced because migration from the border of the wound was very slow"
"STAT1 inhibits chondrocyte proliferation and regulates bone development"
Chondrogenic potential of human mesenchymal stem cells and expression of Slug transcription factor.
"high basal levels of Slug prevent the chondrogenic differentiation of hMSCs, even in the presence of transforming growth factor-β and elevated levels of Sox9."
"In all osteo-differentiated hMSCs, Slug expression significantly increased both at the mRNA and protein level"
"a physical stimulus, such as the pellet 3D culturing technique, may in itself negatively affect expression of Slug. "<-But the 3D cultural technique is not sufficient to reduce Slug expression.
Angptl1 may supress Slug expression and Angptl1 is upregulated by LSJL.
Angiopoietin-like protein 1 suppresses SLUG to inhibit cancer cell motility
"ANGPTL1 suppressed SLUG by inducing expression of miR-630 in an integrin α1β1/FAK/ERK/SP1 pathway–dependent manner."
"ANGPTLs do not bind to the angiopoietin receptor Tie2 or the elated protein Tie1, which indicates that the functional mechanism of ANGPTL proteins may be different from that of angiopoietins"
"ANGPTL1 interacts with integrin α1β1 and represses its downstream signaling"
Chondrogenic potential of human mesenchymal stem cells and expression of Slug transcription factor.
"high basal levels of Slug prevent the chondrogenic differentiation of hMSCs, even in the presence of transforming growth factor-β and elevated levels of Sox9."
"In all osteo-differentiated hMSCs, Slug expression significantly increased both at the mRNA and protein level"
"a physical stimulus, such as the pellet 3D culturing technique, may in itself negatively affect expression of Slug. "<-But the 3D cultural technique is not sufficient to reduce Slug expression.
Angptl1 may supress Slug expression and Angptl1 is upregulated by LSJL.
Angiopoietin-like protein 1 suppresses SLUG to inhibit cancer cell motility
"ANGPTL1 suppressed SLUG by inducing expression of miR-630 in an integrin α1β1/FAK/ERK/SP1 pathway–dependent manner."
"ANGPTLs do not bind to the angiopoietin receptor Tie2 or the elated protein Tie1, which indicates that the functional mechanism of ANGPTL proteins may be different from that of angiopoietins"
"ANGPTL1 interacts with integrin α1β1 and represses its downstream signaling"
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