Height Increase Pages

Thursday, March 25, 2010

CHD4

Chd4 and associated proteins function as corepressors of Sox9 expression during BMP-2-induced chondrogenesis.

"Mouse embryonic fibroblasts (MEFs) differentiate into fully functional chondrocytes in response to bone morphogenetic protein-2 (BMP-2).  We identify proteins differentially expressed during BMP-2-induced chondrogenic differentiation of MEFs. We found 85 downregulated proteins, and Ingenuity Pathways Analysis (IPA) revealed a protein-protein network with chromodomain-helicase-DNA-binding protein 4 (Chd4) in the center. Chromatin immunoprecipitation (ChIP) and nuclease hypersensitivity assays showed that Chd4, interacting with Hdac1/2, cooperates with its related proteins Kap1 and Cbx1 to bind at -207/-148 of the Sox9 promoter. Let-7a targets the 3'UTR of Chd4 to promote chondrogenesis of MEFs. BMP-2 induced the upregulation of let-7a, targeting Chd4 and positively controlling the chondrogenic differentiation of MEFs."

"miR-199* might affect its target gene, Smad1, to regulate early chondrogenic differentiation"

"In hematopoietic stem cells, SNF2-like ATPase Mi-2beta is required for maintenance of multilineage differentiation in the early hematopoietic hierarchy"

"[Chd4 interacts with] Hdac1 and Hdac2 [which bind] to the promoter of Sox9 in vivo"

"BMP-2 induces the upregulation of let-7a, followed by the degradation of its target gene Chd4 and the separation of Hdac1/2 with Chd4. Subsequently, the competitive complex NF-Y-p-300 increasingly binds with the core promoter of Sox9, resulting in an open access for other positive transcription regulators of Sox9"

So inhibiting Chd4 may help in inducing chondrogenic differentiation.

No comments:

Post a Comment